Fig. 5.

Apoptotic effect of TFPNCD treatment in HepG2 cells. The cells were treated with different doses of TFPNCD (10, 20, 40, 80, 160 μg/mL) for 48 h and then stained with annexin V-FITC and propidium iodide (PI) as described in Materials and Methods. The stained cells were analyzed for apoptosis by using a Becton-Dickinson FACS Calibur flow cytometry