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. 2016 Jul 27;11:67. doi: 10.1186/s13000-016-0517-z

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunohistochemical and genetic features of EIMS. a Immunohistochemistry for ALK showed a nuclear membrane staining pattern (400 × original magnification). b Case 3 showed ALK cytoplasmic staining with perinuclear accentuation (200 × original magnification). c Diffuse and strong positivity for desmin (200 × original magnification). d Fluorescence in situ hybridization (FISH) showed split apart of the red and green signals, confirming the presence of ALK rearrangement (1000 × original magnification)