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. Author manuscript; available in PMC: 2016 Jul 27.
Published in final edited form as: Exp Lung Res. 2015;41(9):499–513. doi: 10.3109/01902148.2015.1091053

Fig. 9.

Fig. 9

Fold change in gene expression in HBE-1 cells at 6 hrs, 12 hrs, and 24 hrs following treatment with either HMW heparin (500 µg/mL) or desulfated HMW heparin (500 µg/mL) in the presence of LPS at 1 µg/mL. (A) HMW heparin significantly inhibited mRNA expression of COX-2 in HBE-1 cells stimulated with LPS only at 12 hours after treatment. DS-Heparin did not significantly affect regulation of COX-2 mRNA by LPS at any time point. (B) HMW heparin at 500 µg/mL significantly prevented LPS-induced CXCL8 mRNA expression at 12 and 24 hrs after treatment; 50 µg/mL HMW Heparin was effective only at 12 hrs. Desulfated HMW Heparin (DS-Heparin) had no significant effects on CXCL8 gene expression. * P < 0.05 and ** P < 0.01. Values represent mean ± SE for triplicate analyses, normalized to “LPS only” group of that time point.