Figure 3.
Non-denaturing PAGE-gels confirming the synthesis of multiprotein complexes. (A) Native PAGE-gel showing the successful formation of the complex containing two CaM-ZE proteins assembled on a single DNA double helix. The dominant species observed in the individual lanes of the gel are indicated by Roman numerals. The labeling scheme is as follows: I. DNA scaffold alone; II. assemblies prepared at a 1 to 0.5 ratio of ssDNA scaffold to conjugates; III. a “half” complex composed of the DNA scaffolds and two artificial polypeptides; IV. complete assemblies formed at a 1:1:1 ratio of all assembly components. (B) Gel demonstrating the assembly of multiprotein complexes containing both a GFP-ZE and a CaM-ZE (III), templated on a DNA scaffold possessing two different “overhang” sequences. Partial complexes formed using only one type of protein and corresponding to either CaM-ZE (I), or GFP-ZE (II) are also shown.