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. Author manuscript; available in PMC: 2017 Mar 29.
Published in final edited form as: Stress. 2016 Mar 29;19(2):235–247. doi: 10.3109/10253890.2016.1160280

Figure 7. Effects of 3-PBC in vitro on attenuating alcohol-mediated actions via a benzodiazepine-independent manner.

Figure 7

A) Whole-cell recordings of HEK 293 cells expressing recombinant rat α2β3γ2 GABAA receptors were performed. Currents were normalized to the GABA concentration specific for the receptor subtype EC10 under in vitro conditions. Two concentrations of EtOH [30 mM and 100 mM] in the absence or presence of 1 nM and 30 nM 3-PBC, respectively, were co-applied with 1.5 μM GABA. Asterisks [*] denote P ≤ 0.05 in a two-sided t-test, compared to 0 or 1nM PBC. (B-E) Increasing concentrations of diazepam (B, C) or 3-PBC (D,E) in the absence (white) or presence (gray) of 10 μM Ro15-1788 were co-applied with the receptor specific GABA concentrations at about the EC20. Asterisks (*) denote p < 0.05 in a two-sided t-test comparing diazepam and 3-PBC plus Ro15-1788 to the test compounds alone. Error bars indicate the standard error of the mean (± SEM) for at least four cells.