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. 2016 Jun;11(6):896–897. doi: 10.4103/1673-5374.184455

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Immunofluorescence staining using Iba-1 antibody.

Primary antibody is a mouse monoclonal and fluorescence labeled secondary antibody (yellow) (Alexa Fluor 647 rabbit anti-mouse). Nuclear stain DAPI (blue). The fluorescent signal intensity analyzed using image J software Imaging done using EVOS® FL Cell Imaging System. Iba-1 is a microglial marker. Increased Iba-1 expression in CHME-5/HIV indicating microglial activation in the HIV transfected microglia. DAPI: 4′6-diamidino-2-phenylindole; Iba-1: ionized calcium-binding adapter molecule 1.