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. 2016 Jun;11(6):983–987. doi: 10.4103/1673-5374.184499

Figure 3.

Figure 3

YAP protein expression in NSCs after Y-27632 treatment (western blot assay).

(A) Immunoblots of MAPK, p-MAPK, Akt, p-Akt, ROCKII and YAP. (B) Quantification of p-MAPK/MAPK, p-Akt/Akt, ROCKII and YAP protein expression in NSCs treated with 1.0 μg/mL Y-27632 for 4 hours, normalized against GAPDH and expressed as the ratio to the control group. Western blot assay was performed four times for each measurement. Data are expressed as the mean ± SEM. *P < 0.05, vs. control group (Con) (Student's two-tailed t-test). NSCs: Neural stem cells; (p-)MAPK: (phosphorylated) mitogen-activated protein kinase.