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. Author manuscript; available in PMC: 2016 Dec 16.
Published in final edited form as: N Engl J Med. 2016 Jun 16;374(24):2335–2344. doi: 10.1056/NEJMoa1515520

Table 3.

Results of Pig Necropsy According to Serologic Status at Each Phase of the Cysticercosis Elimination Demonstration Program.*

Phase and Serologic Status Pig Blood Sample Pig Necropsy Sample Pigs with Live Nondegenerated Larval Cysts
number number (percentage)
Phase 1

 Seronegative 197 146 (74.1) 2 (1.4)

 1–2 bands 186 128 (68.8) 4 (3.1)

 3 bands 73 47 (64.4) 10 (21.3)

 4–7 bands 8 5 (62.5) 2 (40.0)

 Total 464 326 (70.3) 18 (5.5)

Phase 2, immediately after intervention

 Seronegative 3024 178 (5.9) 1 (0.6)

 1–2 bands 556 184 (33.1) 0

 3 bands 414 279 (67.4) 4 (1.4)

 4–7 bands 25 17 (68.0) 1 (5.9)

 Total 4019 658 (16.4) 6 (0.9)

Phase 2, 12 months after intervention

 Seronegative 2362 37 (1.6) 0

 1–2 bands 505 143 (28.3) 2 (1.4)

 3 bands 186 114 (61.3) 3 (2.6)

 4–7 bands 20 16 (80.0) 2 (12.5)

 Total 3073 310 (10.1) 7 (2.3)

Phase 3, immediately after intervention

 Seronegative 2532

 1–2 bands 565 53 (9.4) 0

 3 bands 365 235 (64.4) 1 (0.4)

 4–7 bands 68 54 (79.4) 2 (3.7)

 Total 3530 342 (9.7) 3 (0.9)
*

Pig serum samples were assessed by enzyme-linked immunoelectrotransfer blot assay, which uses lentil lectin-bound glycoproteins. Serologic status was based on the number of reactive bands on Western blot analysis.

Necropsy samples of pig muscle or brain tissue were examined for live, nondegenerated cysts filled with clear fluid. Necropsy samples covered most seropositive pigs to maximize the likelihood of detecting infections. Formal viability testing and histopathological analysis were performed in phase 2 only.

Phase 1 necropsy was performed during a culling intervention in which the majority of pigs were removed from six geographically isolated villages in which no chemotherapy or vaccine had been applied previously.