Strains |
|
|
P. aeruginosa strains |
|
|
PAO1 |
ATCC 15692 (wild type, prototroph) |
American Type Culture Collection |
PAO1 PpvdA′-′mCherry |
Wild type with PpvdA::mCherry |
This work |
pvdA mutant |
PAO1 ΔpvdA
|
45 |
pchD mutant |
PAO1 ΔpchD
|
82 |
pvdA pchD mutant |
PAO1 ΔpvdA ΔpchD
|
83 |
PAO1 pvdA pchD PpvdA′-′mCherry |
PAO1 ΔpvdA ΔpchD PpvdA::mCherry |
This work |
fpvR mutant |
PAO1 ΔfpvR
|
This work |
pvdA fpvR mutant |
PAO1 ΔpvdA ΔfpvR
|
This work |
pvdS mutant |
PAO1 ΔpvdS Gmr
|
31 |
hasR phuR mutant |
PAO1 ΔhasR ΔphuR
|
This work |
feoB mutant |
PAO1 ΔfeoB
|
This work |
tonB1 mutant |
PAO1 ΔtonB1
|
This work |
tonB1 feoB mutant |
PAO1 ΔtonB1 ΔfeoB
|
This work |
toxA mutant |
PAO1 phoA wp03q2B07 (mutant 40695) |
Transposon mutant collection of University of Washington |
Escherichia coli strains |
|
|
DH5α |
F′ recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 ϕ80dlacZΔM15 Nalr
|
35 |
S17-1 λpir
|
recA thi pro hsdR(M+ RP4)::2-Tc::Mu::Km Tn7 λpir Tpr Smr
|
84 |
Plasmids |
|
|
pEX18Tc |
Allelic exchange vector pMB1 replicon; oriT+
sacB+
lacZα mob+ Tcr
|
39 |
pDM4 |
Suicide vector; sacBR oriR6K Cmr
|
40 |
pmCherry-1 |
Vector used as template for amplification of the mCherry coding sequence |
Clontech |
Mini-CTX1 |
Promoter-probe vector; Ω-FRT-attP-MCS ori int oriT Tcr
|
42 |
pFLP2 |
FLP recombinase-expressing plasmid; Apr
|
39 |
Mini-CTX1 mCherry |
Tcr
|
This study |
Mini-CTX PpvdA′-′mCherry |
Plasmid to insert a PpvdA-mCherry fusion into the chromosome of P. aeruginosa; Tcr
|
This study |
pDM4ΔfpvR
|
pDM4 derivative carrying the flanking regions of the fpvR coding sequence |
This study |
pDM4ΔhasR
|
pDM4 derivative carrying the flanking regions of the hasR coding sequence |
This study |
pDM4ΔphuR
|
pDM4 derivative carrying the flanking regions of the phuR coding sequence |
This study |
pEXΔfeoB
|
pEX18Tc derivative carrying a Gmr-GFP cassette between the feoB flanking regions |
This study |
pDM4ΔtonB
|
pDM4 derivative carrying the flanking regions of the tonB1 coding sequence |
This study |
pMP220::PfeoA
|
pMP220 derivative carrying a PfeoA::lacZ transcriptional fusion; Tcr
|
31 |
pPZ-toxA
|
pPZ20 derivative carrying a PtoxA::lacZ translational fusion; Cbr
|
26 |
pPZ-prpL
|
pPZTC derivative carrying a PprpL::lacZ transcriptional fusion; Cbr
|
26 |
pUCP18 |
E. coli-Pseudomonas shuttle vector derived from pUC18; ColE1 pRO1600 Apr Cbr
|
41 |
pUCPpvdA
|
pUCP18 derivative carrying the coding sequence of pvdA with its own promoter |
This study |
pUCPpchD
|
pUCP18 derivative carrying the coding sequence of pchD with its own promoter |
This study |
pUCPtonB1
|
pUCP18 derivative carrying the coding sequence of tonB1 with its own promoter |
This study |
pUCPphuRhasR
|
pUCP18 derivative carrying the coding sequences of phuR and hasR with their own promoters |
This study |