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. 2016 Jul 21;84(8):2389–2405. doi: 10.1128/IAI.00121-16

FIG 5.

FIG 5

Analysis of Fur-regulated genes induced in the presence of cysteine. (A) Consensus sequence of the Fur box motif of C. difficile. The sequence logo was created by the alignment of putative Fur-regulated genes induced in the presence of cysteine on the WebLogo website (http://weblogo.berkeley.edu). The height of the nucleotides is proportional to their frequency. (B) Effect of cysteine on the transcript level of fur (CD1287), feoB1 (CD1479), cysK (CD1594), fhuD (CD2878), and CD2992 in 630Δerm and the 630Δerm::fur. The 630Δerm (white boxes) and 630Δerm::fur (black boxes) strains were grown for 10 h in PY or PYC. qRT-PCR results are presented as the ratio of the amount of mRNA (arbitrary units) of each gene in bacterial cells grown in PYC to that of each mRNA in the bacterial cells grown in PY. Data are the averages from at least three independent experiments, and error bars are the standard deviations from the mean values. (C) Aspect of the bacterial pellet of strain 630Δerm grown for 10 h in PY or in PYC. The black precipitate is due to FeS precipitation.