Effect of sigL inactivation on cysteine degradation and pyruvate production. (A) Detection of H2S production in the 630Δerm, 630Δerm::sigL, and 630Δerm::sigL(pDIA6309-sigL) strains grown in PYC by using lead-acetate papers. (B) Quantitative detection of pyruvate in the supernatant of strains 630Δerm, 630Δerm::sigL, and 630Δerm::sigL(pDIA6309-sigL) after 10 h of growth in PY (white boxes) or PYC (black boxes). The statistical analysis was performed by using Mann-Whitney test for all genes. ns, not significant.