TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristic | Gene type | Plasmid or resistance typea | Origin or reference |
|---|---|---|---|---|
| Strains | Background | Knockout or overexpressed gene | Plasmid | |
| 630Δerm | 87 | |||
| M7404 | BI/NAP1/027 | 2 | ||
| M7404 (tcdC+) | BI/NAP1/027 | pDLL17 (tcdC+) | 2 | |
| VPI 10463 | Virginia Polytechnic Institute | |||
| CDIP001 | 630Δerm | CD1287 (fur)::erm | This study | |
| CDIP106 | 630Δerm | CD0278::erm | This study | |
| CDIP107 | 630Δerm | CD2023::erm | This study | |
| CDIP110 | 630Δerm | CD2065::erm | This study | |
| CDIP217 | 630Δerm | CD3176 (sigL)::erm | This study | |
| CDIP342 | 630Δerm | CD3176 (sigL)::erm | pDIA6309 | This study |
| CDIP540 | 630Δerm | CD1594 (cysK)::erm | This study | |
| CDIP656 | 630Δerm | pDIA6456 | This study | |
| CDIP657 | 630Δerm | CD2602::erm | This study | |
| JIR8094 | 88 | |||
| CDIP100 | JIR8094 | CD1064 (ccpA)::erm | 13 | |
| LB-CD15 | JIR8094 | CD1275 (codY)::erm | pBL92 | L. Bouillaut |
| Plasmids | Vector | Cloned gene | Resistance | Origin |
| pDIA5906 | pMTL007 | Intron CD1287 (fur) | Cmr Tmr | This study |
| pDIA6309 | pMTL84121 | CD3176 (sigL) | Cmr Tmr | This study |
| pDIA6450 | pMTL007 | Intron CD0278 | Cmr Tmr | This study |
| pDIA6451 | pMTL007 | Intron CD2065 | Cmr Tmr | This study |
| pDIA6452 | pMTL007 | Intron CD2023 | Cmr Tmr | This study |
| pDIA6453 | pMTL007 | Intron CD3176 | Cmr Tmr | This study |
| pDIA6454 | pMTL007 | Intron CD2602 | Cmr Tmr | This study |
| pDIA6455 | pMTL007 | Intron CD1594 (cysK) | Cmr Tmr | This study |
| pDIA6456 | pRPF185 | Antisense CD3029 (malY) | Cmr Tmr | This study |
a
Cm, chloramphenicol; Tm, thiamphenicol; Erm, erythromycin.