TABLE 1.
Strain or plasmid | Characteristic | Gene type | Plasmid or resistance typea | Origin or reference |
---|---|---|---|---|
Strains | Background | Knockout or overexpressed gene | Plasmid | |
630Δerm | 87 | |||
M7404 | BI/NAP1/027 | 2 | ||
M7404 (tcdC+) | BI/NAP1/027 | pDLL17 (tcdC+) | 2 | |
VPI 10463 | Virginia Polytechnic Institute | |||
CDIP001 | 630Δerm | CD1287 (fur)::erm | This study | |
CDIP106 | 630Δerm | CD0278::erm | This study | |
CDIP107 | 630Δerm | CD2023::erm | This study | |
CDIP110 | 630Δerm | CD2065::erm | This study | |
CDIP217 | 630Δerm | CD3176 (sigL)::erm | This study | |
CDIP342 | 630Δerm | CD3176 (sigL)::erm | pDIA6309 | This study |
CDIP540 | 630Δerm | CD1594 (cysK)::erm | This study | |
CDIP656 | 630Δerm | pDIA6456 | This study | |
CDIP657 | 630Δerm | CD2602::erm | This study | |
JIR8094 | 88 | |||
CDIP100 | JIR8094 | CD1064 (ccpA)::erm | 13 | |
LB-CD15 | JIR8094 | CD1275 (codY)::erm | pBL92 | L. Bouillaut |
Plasmids | Vector | Cloned gene | Resistance | Origin |
pDIA5906 | pMTL007 | Intron CD1287 (fur) | Cmr Tmr | This study |
pDIA6309 | pMTL84121 | CD3176 (sigL) | Cmr Tmr | This study |
pDIA6450 | pMTL007 | Intron CD0278 | Cmr Tmr | This study |
pDIA6451 | pMTL007 | Intron CD2065 | Cmr Tmr | This study |
pDIA6452 | pMTL007 | Intron CD2023 | Cmr Tmr | This study |
pDIA6453 | pMTL007 | Intron CD3176 | Cmr Tmr | This study |
pDIA6454 | pMTL007 | Intron CD2602 | Cmr Tmr | This study |
pDIA6455 | pMTL007 | Intron CD1594 (cysK) | Cmr Tmr | This study |
pDIA6456 | pRPF185 | Antisense CD3029 (malY) | Cmr Tmr | This study |
Cm, chloramphenicol; Tm, thiamphenicol; Erm, erythromycin.