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(A) Time lapse images of Blastocystis ST7-B cells treated with 100 μg/ml LL-37 (top) and controls (bottom). Images were taken every 5 min. Arrows point to cells undergoing lysis. Cells are lysed as early as 5 min after the addition of LL-37 (white arrow). (B) Time course lysis experiments using PI staining and flow cytometry were also done. Graphs show that the membrane-disrupting effect of LL-37 is faster among ST4-WR1 than among ST7-B cells.
