Blastocystis ST7-B cells (A, C, and E) have thicker and denser surface coats (arrows) than do ST4-WR1 cells (B, D, and F), as shown by using India ink as a negative stain for bright microscopy (A and B) and transmission electron microscopy (C, D, E, and F). (G) Images from TEM were used to measure the thickness of the surface coat. The surface coat of the ST7-B isolate is more than double the thickness of that of the ST4-WR1 isolate. *, P < 0.05.