FIG 1.
CLH001 is highly attenuated in BALB/c mice compared to strain CSM001 and exhibits increased safety in comparison to strains TMM001 and CLH002 at day 21 postinfection. (A) Percent survival of BALB/c mice (n = 8) following i.n. challenge with 1.5 × 104 CFU of CSM001 (●), TMM001 (■), CLH002 (◆), or CLH001 (▲) at 21 days postinfection. (B to D) Colonization of mouse lungs (B), livers (C), and spleens (D) (n = 3) at day 2 (▲) and day 21 (■) postinfection with 1.5 × 104 CFU of TMM001, CLH002, or CLH001. The limit of detection was 10 CFU/organ (horizontal dotted line). (E to G) Results of comparisons of histopathological scores for lungs (E), livers (F), and spleens (G) of PBS-treated mice versus mice infected with TMM001, CLH002, or CLH001 (n = 3) at 21 days postinfection. NS, not significant.