FIG 5.

Iron- and zinc-mediated suppression of EsxH impair intracellular survival. BMDMs were infected with M. tuberculosis that had been pregrown for 3 days in CS medium containing iron or zinc as indicated (A and B) or in 7H9 (C and D) at a multiplicity of infection of 3. (A to C) CFU were enumerated at 4, 24, and 72 hpi, and data are expressed as the percent bacterial survival at 24 hpi (CFU at 24 hpi/CFU at 4 hpi) ± the SEM and 72 hpi (CFU at 72 hpi/CFU at 4 hpi) ± the SEM from three experiments, with five experimental replicates each. (D) BMDMs were infected with wild-type M. tuberculosis, ΔesxH, and ΔesxH::esxH strains, and the CFU were enumerated 72 and 96 hpi from five replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student t test). n.s., not significant.