Skip to main content
. Author manuscript; available in PMC: 2016 Jul 27.
Published in final edited form as: Cancer Prev Res (Phila). 2012 May;5(5):788–797. doi: 10.1158/1940-6207.CAPR-12-0004-T

Figure 6. Over-expression of Akt and c-Myc blocked the suppressive effect of CAPE on proliferation and CAPE blocked AR transcriptional activity.

Figure 6

(A) 104-S cells overexpressing Akt1 or c-Myc were treated with increasing concentrations of CAPE for 96 hr and analyzed by 96-well proliferation assay. (B) Protein expression of Akt, c-Myc, Akt1, Akt2, Akt3, phospho-Akt S473, phospho-Akt T308, and α-tubulin were assayed by Western blotting of 104-S cell lines overexpressing c-Myc or Akt1 prior to CAPE treatment. (C) RWPE-1 cells were treated with increasing concentrations of CAPE for 96 h to determine suppressive effect of CAPE on normal prostate epithelial cells. (D) Protein expression of total Akt, phospho-Akt S473, phospho-Akt T308, phospho-GSK3β S9, and β-actin in RWPE-1 cells treated with 0, 3, and 10 μM CAPE for 96 h was assayed by Western blotting.