Fig 4.

Blocking KIR3DL1-h interaction with Bw4-80I rescues natural killer cells (NK cells) from inhibition. Peripheral blood mononuclear cells from healthy donors were stimulated with 3F8 and KIR3DL1 ligand-matched cell line SKNBE(1)n with or without interferon-gamma (IFN-γ) pretreatment. Effect of addition of KIR3DL1 monoclonal antibody (DX9) and anti–HLA-B-C antibody on CD107a degranulation was evaluated in NK cells that exclusively express KIR3DL1-n (blue bars), KIR3DS1 (gold bars), and KIR3DL1-h (gray bars). Data represent two independent trials with four to six donors per group, per trial.