Table 1.

Ethogram used when scoring behaviour in CBA mice pre and post vasectomy. * Indicates behaviours that were categorised as ‘pain behaviours’.

Behaviour	Definition
Belly press*	Pressing of abdomen toward cage floor
Flinch*	Small movement involving whole body
Raised tail*	When walking, tail is lifted from
Rear Leg Lift*	Lifting one rear leg straight out behind
Stagger*	Partial loss of balance when walking
Twitch*	Rapid contraction of back muscles
Writhe*	Contortion of abdominal muscles
Full rear	Standing on rear legs
Partial rear	Standing on rear legs to half stretch
Grooming	Grooming of head, face, back, abdomen, limbs or tail