Figure 6.

Localization of mRNA in Arabidopsis thaliana meristematic root cells. Localization of mRNA of ADH1 before (A, B) and after 1 h hypoxia (C, D). Localization of mRNA of MSR B8 before (F, G) and after 1 h hypoxia (H, I). Double labeling of MSR B8 transcripts (K) and Sm-proteins (L) in meristematic root cells after 1 h hypoxia (K–M). In situ hybridization to mRNA of RPB2 under normoxia (N–O) and after 1 h hypoxia (P–Q). Simultaneous localization of the mRNA of RPB2 (S) and core spliceosomal Sm proteins (T) in cells after 1 h hypoxia revealed strong colocalization in Cajal bodies (S–U) (arrowheads). Merging with DAPI staining (B, D, G, I, M, O, Q, U). Bar, 20 µm. N- nucleus, Cy- cytoplasm, arrows- Cajal bodies. Histogram shows quantitative analysis of ADH1 (E), MSR B8 (J), RPB2 (U) in the nuclei (N) and cytoplasm (C) under normoxia and hypoxia Arabidopsis thaliana the meristematic roots cells. The tables under plot present the significant differences between groups, with testing probability below the value shown in the table (P<), α = 0.05. In the column heading, the number of analyzed cells in each stage is presented in brackets. ns - nonsignificant differences between groups.