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. 2015 Nov 20;13(5):486–499. doi: 10.1080/15476286.2015.1110673

Figure 7.

Figure 7.

RNase E-enriched protein fraction of S. meliloti. RNase E of S. meliloti was enriched by a method used for purification of bacterial degradosome complexes.32-35 Fractions 10 of the glycerol gradient shown on Fig. S10 was analyzed by 8 % glycine SDS PAGE (A) and 16 % tricine SDS-PAGE (B) and silver staining. Proteins identified by MALDI-TOF-MS are marked with asterisks. The corresponding genes are given in brackets. PDC, pyruvate dehydrogenase complex. To exclude RNA-mediated co-purification of proteins, 2 gradients were ran in parallel with (+) or without (-) RNase A (indicated above the panels). M, protein marker, sizes are indicated in kDa.