Skip to main content
. 2016 Mar 22;13(5):500–510. doi: 10.1080/15476286.2016.1164365

Figure 4.

Figure 4.

MiR-26 family targets INSIG1. (A) The correlation between expression level of miR-26a/b and INSIG1 in 24 mid-lactation mammary gland tissue samples. (B) Regulation of INSIG1 mRNA level by miR-26a/b mimic. (C) Regulation of INSIG1 protein level by miR-26a/b mimic and inhibitor. (D) MiR-26 family site in INSIG1 3’UTR and the construction of the luciferase (Luc) expression vector fused to the INSIG1 3’UTR. Bta: Bovine; WT, Luc reporter vector with the WT INSIG1 3’UTR (2224 bp to 2612 bp); MU, Luc reporter vector with the mutation at miR-26 family site in INSIG1 3’UTR. (E) Induction or suppression of Luc activity with the WT or MU of the INSIG13’UTR by miR-26 family mimic or inhibitor. GMEC were co-transfected with 120 nM oligonucleotides (60 nM each), WT and MU. The Luc activity was measured at 48 h posttransfection. All experiments were performed in triplicate and repeated 3 times (n = 9). Values are presented as mean + SEM, *, P < 0.05; **, P < 0.01.