. 2016 Mar 22;13(5):500–510. doi: 10.1080/15476286.2016.1164365

Table 1.

miR-26a/b function synergistically with their host genes to regulate FA synthesis gene expression.

Gene symbol	anti-miR-C/NC	anti-miR-26a/NC	anti-miR-26b/NC	anti-miR-26a/b	siCTDSP1/anti-miR-C	siCTDSP2/anti-miR-C	siCTDSPL/anti-miR-C	anti-miR-26a/siCTDSP2	anti-miR-26a/siCTDSPL	anti-miR-26b/siCTDSP1
ACACA	1.30 + 0.08	0.83 + 0.01^*	0.89 + 0.03^*	0.79 + 0.02^*	1.00 + 0.03	0.81 + 0.04^*	1.00 + 0.07	0.76 + 0.02^#	0.76 + 0.04	0.86 + 0.10
AGPAT6	1.06 + 0.05	0.95 + 0.03	1.04 + 0.09	0.94 + 0.08	1.00 + 0.10	0.91 + 0.06	1.07 + 0.05	1.02 + 0.02	1.24 + 0.05	1.05 + 0.06
CD36	0.82 + 0.01	0.71 + 0.06	0.59 + 0.03^*	0.55 + 0.06^*	1.00 + 0.05	0.95 + 0.05	0.86 + 0.04	0.78 + 0.05^$	0.46 + 0.06^#,^$	0.54 + 0.04^$
DGAT1	1.48 + 0.09	0.99 + 0.01^*	0.87 + 0.03^*	0.73 + 0.02^**	1.65 + 0.07	1.08 + 0.08^*	1.00 + 0.07^*	1.05 + 0.05	0.95 + 0.01	0.84 + 0.02^$
FASN	1.76 + 0.09	1.42 + 0.10	0.90 + 0.03^**	0.82 + 0.02^**	1.10 + 0.04^*	1.49 + 0.11	1.00 + 0.10^*	1.03 + 0.05^#,^$	1.23 + 0.04	0.81 + 0.05^$
GPAM	1.40 + 0.06	0.73 + 0.04^**	0.89 + 0.01^*	0.96 + 0.03^*	1.00 + 0.09	0.81 + 0.02^*	1.00 + 0.06	0.78 + 0.09	0.71 + 0.03	0.73 + 0.03^#
INSIG1	1.12 + 0.13	2.33 + 0.11^*	3.50 + 0.03^**	3.94 + 0.16^**	3.13 + 0.04	2.29 + 0.05	2.54 + 0.08^**	2.70 + 0.10^$	2.97 + 0.13	3.15 + 0.08
LPIN1	1.30 + 0.05	0.85 + 0.09^*	0.84 + 0.04^*	0.84 + 0.04^*	1.00 + 0.01	1.16 + 0.09	1.19 + 0.02	0.74 + 0.01^$	0.86 + 0.03	0.84 + 0.03
LXRA	1.19 + 0.06	0.78 + 0.02^*	0.81 + 0.03^*	0.76 + 0.03^*	0.84 + 0.09	0.80 + 0.06^*	1.00 + 0.01	0.74 + 0.02	0.77 + 0.03^$	0.69 + 0.03^$
PLIN2	1.16 + 0.07	1.00 + 0.09	1.05 + 0.04	0.83 + 0.01^*	0.90 + 0.06	0.77 + 0.06^*	0.81 + 0.06^*	1.05 + 0.09	0.71 + 0.01	0.79 + 0 09
PNPLA2	1.24 + 0.08	1.06 + 0.11	0.99 + 0.04	0.90 + 0.04^*	0.82 + 0.09	2.81 + 0.16^**	1.69 + 0.09	1.51 + 0.11^#	2.56 + 0.10^#,^$	1.70 + 0.04^#,^$
PPARG	0.82 + 0.10	0.61 + 0.06^**	0.54 + 0.03^**	0.60 + 0.02^**	0.61 + 0.03^*	0.98 + 0.02	0.86 + 0.07	0.56 + 0.02^$	0.69 + 0.07	0.48 + 0.06
SCD1	1.24 + 0.07	0.64 + 0.05^**	0.75 + 0.01^*	0.57 + 0.03^**	1.00 + 0.05	0.73 + 0.01^**	1.13 + 0.07	0.87 + 0.07	0.87 + 0.03	0.89 + 0.06
SREBF1	1.62 + 0.03	1.45 + 0.07	0.87 + 0.08^**	0.71 + 0.03^**	0.96 + 0.06^**	1.03 + 0.07^*	1.00 + 0.02^*	0.91 + 0.03^#	0.94 + 0.09^#	0.71 + 0.03

GMEC were co-transfected with 120 nM oligonucleotides (60 nM each) and after 48 h post-transfection, the ACACA, AGPAT6, CD36, DGAT1, FASN, GPAM, INSIG1, LPIN1, LXRA, PLIN2, PNPLA2, PPARG, SCD1 and SREBF1 expression levels were quantified by quantitative real-time PCR. All experiments were performed in triplicate and repeated 3 times (n = 9). Values are presented as mean ± SEM;

P < 0.05,

^**

P < 0.01 vs anti-miR-C/NC,

P < 0.05 vs anti-miR-26a/NC or anti-miR-26b/NC,

P < 0.05 vs siCTDSP1/anti-miR-C or siCTDSP2/ anti-miR-C or siCTDSPL/ anti-miR-C.