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. 2016 May 5;13(6):593–604. doi: 10.1080/15476286.2016.1184384

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 1. — P-type pentatricopeptide repeat protein-modulating editing (PPME) protein is a P-type pentatricopeptide repeat (PPR) protein essential for normal Arabidopsis growth and development. (A) The PPME gene structure and its encoded P-type PPR protein harboring 16 PPR (35 amino acids) motifs is shown; ppme-1 was generated by T-DNA insertion in the coding region. (B) RT-PCR analysis of constitutive PPME expression, of a null homozygous ppme-1 mutant and of transformed PPME in T3 transgenic lines complemented with CaMV 35S promoter-driven (35S::PPME-GFP/ppme−/−, comp1) or PPME native promoter-driven (PPMEg-GFP/ppme−/−, comp2) PPME genomic fragments in a homozygous ppme-1 background. (C) Fourteen-day-old heterozygous ppme+/− germinated F2 seedlings grown on solid MS medium. (D) Fourteen-day-old homozygous ppme−/− seedlings with stunted vegetative growth. (E) Forty-day-old homozygous ppme−/− plants survived on solid MS medium for 21 d before being transferred to soil. (F-G) Fourteen-day-old seedlings (F) and 40-day-old (G) plants from wild-type, homozygous ppme−/−, comp1, and comp2 plants. (H-I) Siliques of homozygous ppme−/− showed an abortion phenotype (H), with shrunken seeds and reduced viability (I). Scale bars = 0.5 cm in C-G; 2 mm in H; and 500 μm in I.