Table 1.

Genotyping of heterozygous ppme F2 generation. In total, 236 seeds harvested from heterozygous ppme plants were germinated in soil for 1 month. Genotyping revealed that 34% (80) and 66% (156) of the progeny were wild-type and heterozygous plants, respectively, and no homozygous ppme plants were found.

Genotype	Wild-type	Heterozygous	Homozygous	P value* (2:1)
ppme heterozygous F2	80	156	0	0.853923

^*The p-value indicates that the segregation ratio was 2:1 (heterozygous to wild-type) according to the chi-square test.