Fig. 3.

Viability (left) and viable cell number (right) of samples experiencing PS in 6 ml vials, day 1, 2, and 3 post-thaw (top, middle, and bottom respectively). All sets recover from a nadir at 1 day post thaw, By 3 days post-thaw, viability is significantly worse in the top (5th) quintile compared to viability in any of 1st (bottom) to 3rd quintiles. No significant difference in viability is observed between quintiles 1–4, 3 days post thaw. Viable cell number 3 days post thaw is significantly worse in the 5th quintile compared to all other locations. All n = 5 ± SD. Significance defined as P < 0.01 using an unpaired Student’s t-test.