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. Author manuscript; available in PMC: 2017 May 7.
Published in final edited form as: Anal Methods. 2016 Apr 7;8(17):3458–3465. doi: 10.1039/C6AY00698A

Fig. 4.

Fig. 4

a) Michaelis-Menten model of H3K9(Ac) with 0.5 μM SIRT1. The fitted Km value is 22 μM. b) Linear reaction within 2 h. c) Linear calibration curve of 0 to 25 μM H3K9. Intensity ratio of H3K9 to H3K9* was measured (n = 3).