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. Author manuscript; available in PMC: 2016 Jul 27.
Published in final edited form as: Nat Cell Biol. 2014 Jul 27;16(8):792–803. doi: 10.1038/ncb3007

Figure 8. Treacle-dependent NBS1 recruitment into the nucleoli mediates rRNA silencing in response to DNA damage.

Figure 8

(a) rRNA transcription was measured by EU labeling after transfection of cells with Treacle shRNA and in the presence of 2 Gy of IR. Error bars represent S.E.M. (3 independent experiments; n represents number of cells analyzed: Con: n =110, 100, 90; shTreacle: n =127, 197, 91; 2 Gy: n = 55, 89, 73; 2 Gy + shTreacle: n = 256, 48, 71). (b) rRNA transcription was measured by EU labeling in the presence of Actinomycin D. Error bars represent S.E.M. (4 independent experiments; n represents number of cells analyzed: Mock: n = 4252, 3848, 3310, 4865; ActD: n = 4113, 3936, 4314, 3975). (c) rRNA transcription was measured by EU labeling after transfection of cells with NBS1-GFP-NOLS. Quantification of signal intensities using ImageJ plot profile (2 independent experiments; one representative image shown). Scale bar: 10 µm. (d) Model of Treacle-NBS1-mediated trans-compartmental communication between sites of DNA breaks and the nucleoli.