Figure 6.

Therapeutic efficacy as measured by systemic parasitemia. Mice were infected with T. cruzi, and then immunized at days 7 and 14 post infection, as shown by arrows (↓). The vaccine was compared to controls lacking the CpG ODN (lacking adjuvant control) or the Tc24 protein (lacking antigen control), both delivered in the nanoparticle delivery system, as well as a sham vaccine group. Parasitemia in the blood was measured twice weekly by quantitative real-time PCR throughout the acute phase of infection. Data are plotted as median and interquartile range (n = 10). Significance was calculated using Kruskal-Wallis test with Dunn's correction for multiple comparisons; *p < 0.05 when comparing the vaccine to the sham.