Figure 8.

Therapeutic efficacy as measured by cardiac inflammatory infiltrate Mice were infected with T. cruzi, and then immunized at days 7 and 14 post infection. At 50 days post infection, the vaccine was compared to controls lacking the CpG ODN (lacking adjuvant control) or the Tc24 protein (lacking antigen control), both delivered in the nanoparticle delivery system, as well as a sham vaccine group. Quantification of the percentage of area occupied by inflammatory cell nuclei in the cardiac tissue over 5 representative fields of view (100x). Each point represents an individual animal (n = 8–9); median values are denoted by horizontal lines. Significance was assessed by the Kruskal-Wallis test with the post-hoc comparison of all groups to the sham vaccine control using the Mann-Whitney test with Bonferroni adjustment.