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. 2016 Jul 25;34(34):4017–4024. doi: 10.1016/j.vaccine.2016.06.043

Fig. 4.

Fig. 4

Correlation of Treg frequency and antigen specific IL-10 expression. Mice (n = 5) were immunized i.n. with ova-NE or ova-PBS. 42 days after immunization, splenocytes were harvested and stimulated with ova, or ova-I or ova-II peptides for 48 h. (A) IL-10 secretion in cell culture determined by Milliplex. (B) Splenic Treg frequency and production of IL-10 after stimulation with ova were plotted for each individual mouse to demonstrate the correlation between Tregs and IL-10 expression for NE immunized mice.