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. 2016 Jul 27;11(7):e0159810. doi: 10.1371/journal.pone.0159810

Table 1. qRT-PCR validated miRNAs and their targets in the PH6WC and PH4CV lines.

miRNA Name Target Transcript Transcript Annotation Homologous Genes and Biological Function
zma-miR156a,b,c,d,e,f,g,l-5p GRMZM2G126827_T01 SBP12; SBP transcription factor family protein SPL10, SPL11; regulation of timing of transition from vegetative to reproductive phase [46]
GRMZM2G156621_T01 SBP transcription factor family protein SPL10, SPL11; regulation of timing of transition from vegetative to reproductive phase [46]
zma-miR159c_L-1R-1-3p GRMZM2G020805_T01 TCP family transcription factor TCP2; positive regulation of development, heterochronic [48]
zma-miR167g_R+1,h_R+1,i_R+1-5p GRMZM2G042623_T01 Pumilio-family RNA binding protein APUM1; regulation of translation [49]
GRMZM2G042623_T02 Pumilio-family RNA binding protein APUM1; regulation of translation [49]
zma-miR171c_1ss4CT,d,e,n_R+1_1ss12CT-3p GRMZM2G037792_T01 GRAS79; GRAS transcription factor SCL6; regulation in flowering time [50–51]
GRMZM5G825321_T01 GRAS transcription factor SCL6; regulation in flowering time [50–51]
GRMZM5G825321_T02 GRAS transcription factor SCL6; regulation in flowering time [50–51]
zma-miR319a_R+1,b_R+1,c_R+1,d_R+1-3p GRMZM2G020805_T01 TCP family transcription factor TCP2; positive regulation of development, heterochronic [48]
zma-miR396a,b-5p GRMZM2G024293_T01 XPA-binding protein 1 QQT2; embryo development ending in seed dormancy [53]
GRMZM2G024293_T03 XPA-binding protein 1 QQT2; embryo development ending in seed dormancy [53]
GRMZM2G034876_T01 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G034876_T02 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G034876_T03 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G041223_T01 GRF6; GRF transcription factor GRF6; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G045977_T01 GRF13; GRF transcription factor GRF3; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G129147_T01 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G129147_T02 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G149543_T01 - -
zma-miR396c_L-1 GRMZM2G034876_T01 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G034876_T02 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G034876_T03 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G041223_T01 GRF6; GRF transcription factor GRF6; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G045977_T01 GRF13; GRF transcription factor GRF3; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G129147_T01 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
GRMZM2G129147_T02 GRF transcription factor GRF5; regulation of cell expansion in leaf and cotyledons tissues [52]
zma-miR444a_1ss12TC,b_1ss12TC GRMZM2G001024_T01 RING/U-box superfamily protein EDA18; pollen and embryo sac development [54–55]
GRMZM2G001024_T02 RING/U-box superfamily protein EDA18; pollen and embryo sac development [54–55]
GRMZM2G001024_T03 RING/U-box superfamily protein EDA18; pollen and embryo sac development [54–55]
zma-miR827_R-1-3p GRMZM2G003992_T01 - -
zma-miR166b,d,e,g,h,i-3p GRMZM2G038198_T01 START domain containing protein ATHB9; determination of adaxial-abaxial polarity in ovule primordium [56]