Fig 5. Transfer of convalescent serum or B cells from 17D-204 immunized mice to naïve mice partially protects against challenge with wtYFV.

5–6 week old mice were immunized s.c. with 17D-204. (A) serum was harvested and screened by PRNT₈₀ for 17D-204 and Ang71 neutralizing antibody at the indicated days post immunization [***, p = 0.0001 paired t-test]. (B-D) On d21 following immunization, spleens, lymph nodes and sera were collected. CD19+ cells were enriched according to S2 Fig and adoptively transferred into naive 8 week old mice. Some mice received passive transfer of sera and total splenocytes from 17D-204 or mock vaccinated animals. Twenty-four hours after the transfer, mice were challenged with Ang71 and monitored daily for (B) cumulative weight loss (C) maximum weight loss and (D) survival. Weight is expressed as a percentage of weight on d0 of challenge [ns, not significant; **, p≤0.005; ***, p≤0.0005; One-way ANOVA with Tukey's multiple comparisons test]. Refer to Table 1 for statistics comparing percent of survival and numbers of animals. These data are the combined results of two independent experiments.