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. 2016 Jul 27;12(7):e1005793. doi: 10.1371/journal.ppat.1005793

Fig 7. VdCrz1 signaling is involved in penetration peg development and pathogenicity of V. dahliae.

Fig 7

A. Expression analysis of the VdCrz1 signaling-related gene in V592, VdΔnoxb, VdΔpls1 and VdΔcrz1(left panel), as well as in VdΔnoxb and VdΔpls1 with OH·-treatment (right panel). Total RNA was isolated from hyphae grown on the cellophane membrane for 2 days. Results were obtained from at least three biological repeats. Standard deviations are indicated by the error bars. The asterisks indicate significant differences (P<0.05, one-way ANOVA). B. Localization of VdCrz1::GFP in V592, VdΔnoxb and VdΔpls1, and VdΔnoxb/VdNoxB and VdΔpls1/VdPls1 which were grown on MM medium overlaid with the cellophane membrane for 2 days. C. Delayed penetration of VdΔcrz1 mutant hyphae through the cellophane membrane. Penetration of V592 occurred at 3 dpi, whereas penetration of VdΔcrz1 mutant hyphae occurred at 4 dpi. Penetration of VdΔcrz1/VdCrz1::GFP complemented strain restores at 3 dpi. D. Delayed development and decreased virulence in VdΔcrz1-infected cotton plants and restored pathogenicity in VdΔcrz1/Crz1::GFP-infected cotton plants.