Table 1. Physical Characterization of Head-only RSV F Immunogens.
Construct | Oligomeric Statea | Yieldb (mg/L) | Antibody affinity KD (nM) | Physical Stabilityc | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Temperature (°C) | pH | Osmolarity (mM) | Freeze-thaw | ||||||||||
D25 | AM22 | Motad | 50 | 70 | 90 | 3.5 | 10 | 10 | 3000 | ||||
DS-Cav1e | Trimer | 1.9 | 0.15 | <0.01 | 0.04 | 0.9 | 0.0 | ND | 0.8 | 0.9 | 1.0 | 0.8 | 0.7 |
i-273 | Monomer | 0.3 | 33.3 | 13.6 | 1360 | 1.0 | 1.1 | 1.3 | 1.0 | 1.0 | 0.8 | 0.9 | 1.1 |
i-693f | Dimer | 0.5 | 9.9 | 20.3 | 6.5 | 0.8 | 1.2 | 0.1 | 0.8 | 0.8 | 1.4 | 1.4 | 0.6 |
i-210 | Trimer | 5.5 | 0.7 | 4.2 | 761 | 0.9 | 1.0 | 0.2 | 0.9 | 0.9 | 1.3 | 1.2 | 0.1 |
i-447 | Trimer | 3.6 | 0.7 | 1.2 | 0.5 | 1.0 | 1.0 | 0.1 | 1.0 | 1.0 | 1.0 | 0.5 | 0.5 |
a Assessed by gel filtration.
b Yield following gel filtration.
c Fractional D25 reactivity after 60 minutes at specified conditions or 10 cycles of freeze-thaw.
d Mota, motavizumab.
e Values from reference [16].
f Maximum initial fractional D25 reactivity is reported for physical stability.
ND, not determined.