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. 2016 Jul 27;12(7):e1006216. doi: 10.1371/journal.pgen.1006216

Fig 5. Increased expression of Ycg1 delays entry into S-phase.

Fig 5

(A) 5-fold dilutions of strains with the indicated genotypes (YTD33, YTD148, YTD336) were plated on YPD and incubated at the indicated temperatures until the colonies in the wild-type strain were of similar size. (B-C) Wild-type (YTD276), ycg1-K977A (YTD290), and TEFp-YCG1 (YTD361) strains were synchronized in G1 as in Fig 4D and samples were fixed at 7.5 minute intervals after release to measure the percentage of budded cells (B), and progression through S phase by flow cytometry (C). (D) Wild-type (YTD276), ycg1-K977A (YTD290), and TEFp-YCG1 (YTD361) strains were synchronized in metaphase with a MET3p-CDC20 shut-off allele then released into the cell cycle and samples fixed at 7.5 minute intervals after release. Progression into G1 phase was measured by flow cytometry.