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. 2016 Jul 27;12(7):e1006216. doi: 10.1371/journal.pgen.1006216

Fig 6. Cell cycle-regulation of Ycg1 limits condensin complex formation in G1 phase.

Fig 6

YCG1 (YTD302, YTD394, YTD396) and TEFp-YCG1 (YTD355, YTD395, YTD397) strains were synchronized in mitosis with a MET3p-CDC20 shut-off allele, or in G1 by releasing from the mitotic arrest into medium containing alpha-factor for 2 hours. Condensin complexes were then immunoprecipitated from arrested cells with antibodies against a 3V5 tag on Brn1 (A), Ycs4 (B), or Smc2 (C), and Ycg1 association with each subunit was assayed by Western blot for the 3HA tag on Ycg1. In all experiments, a strain lacking a V5 tag (YTD276) was used as a negative control. Flow cytometry data verifying cell-cycle arrest is shown in S6 Fig.