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. 2016 Jul 27;12(7):e1005018. doi: 10.1371/journal.pcbi.1005018

Fig 6. Transcription factor activity in macrophages under M1 (+LPS) or M2 (+IL-4/IL-13) stimulating conditions.

Fig 6

Primary murine BMDMs were stimulated with (A) LPS (50 ng/ml) or (B) IL-4/IL-13 (25 ng/ml each) for the times indicated and whole cellular protein extracts were prepared. 30 μg of protein/lane were subjected to immunoblot analysis using antibodies specific for (A) STAT3-Tyr705, STAT3, p65-Ser536, p65, p38-Thr180/Tyr182, p38 or for (B) STAT6-Tyr641, STAT6, p65-Ser536, p65, p38-Thr180/Tyr182, p38 and GAPDH for loading control. Representative data for at least three independent experiments are shown.