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Neurons (10–12 DIV) were transfected with the Syn-promoter driven GFP-FMRP expression vector. Time-lapse video microscopy showing small puncta emerging from large cargo-like structures and moving out of the dendrite main axis to reach the spine head. Arrows in the top insets indicate the anterograde flow movements. Arrow heads in the bottom image point to puncta emerging from the anterograde flow.
