Figure 5.

Inhibition of PI3K/Akt and Erk1/2 pathways partially attenuates the reduction of the ER stress by bFGF in primary hippocampal neurons. (A) Primary hippocampal neurons were treated with or without the specific inhibitors LY294002 (20 μM) and U0126 (20 μM). Cell lysates were then analyzed by Western blotting for the expression of p-Akt, Akt, p-Erk, Erk, GRP78, XBP-1, ATF6, cleaved-caspase-12, and TH. Bar diagrams of (B) p-Akt/Akt and p-Erk/Erk; (C) GRP78, XBP-1, and ATF6; (D) cleaved caspase-12 and CHOP; and (E) TH from three Western blot analyses. *P < 0.05 versus control group, ^#P < 0.05 versus 6-OHDA group (n = 3).