Figure 7.

Neurogenesis in the dentate gyrus area was evaluated by anti-DCX (doublecortin) immunostaining five weeks after the BCCAo. (A-F) Representative images of DCX immunoreactivity in ischemic animals (A, B, D, E), and sham-treated controls (C, F). (D-F) Higher magnification images of the areas delineated with a rectangle in (A-C). Str. gr., stratum granulare (the granular layer); str. mol., stratum moleculare (the molecular layer); arrowheads show post-mitotic immature neurons. Scale bar in (A-C) equals 200 μm; scale bar in (D-F) equals 80 μm. (G) The average number of DCX-positive cell profiles per section. P values are derived from three-way ANOVA followed by pairwise comparisons with Bonferroni’s adjustment; * p < 0.05 in comparison to the Nfkb1-wt animals, ## p < 0.01 main sex effect. (H, I) Correlations between the average number of DCX+ cells and total CD45 immunoreactivity in the hippocampus (two hippocampi per animal analyzed separately) in female (H) and male animals (I). Spearman’s rho (nonparametric) correlation coefficient R was calculated separately for hippocampi with CD45 immunoreactivity < 9.5% (< 10% in the case of females) shown on the left side of the correlation plot and CD45 immunoreactivity > 9.5% shown on the right.