Table 2.
Primer sequences for mouse genes used in quantitative real-time PCR.
| Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
| Acaa2 | ATGTGCGCTTCGGAACCAAA | CAAGGCGTATCTGTCACAGTC |
| Acetyl-coenzyme A acyltransferase 2/short chain-specific 3-ketoacyl-CoA thiolase (mitochondrial) | ||
| Acadl | TGCCCTATATTGCGAATTACGG | CTATGGCACCGATACACTTGC |
| Acyl-coenzyme A dehydrogenase, long chain | ||
| Acadvl | TGACCTTGGTGTTAGCGTTAC | CTGGGCCTTTGTGCCATAGAG |
| Acyl-coenzyme A dehydrogenase, very long chain | ||
| Acox1 | TCGAAGCCAGCGTTACGAG | ATCTCCGTCTGGGCGTAGG |
| Acyl-CoA oxidase 1, palmitoyl | ||
| Acsl1 | ACCAGCCCTATGAGTGGATTT | CAAGGCTTGAACCCCTTCTG |
| Acyl-CoA synthetase, long chain family member 1 | ||
| Angptl4 | TCCAACGCCACCCACTTAC | TGAAGTCATCTCACAGTTGACCA |
| Angiopoietin-like 4 | ||
| Cpt1a | CTATGCGCTACTCGCTGAAGG | GGCTTTCGACCCGAGAAGA |
| Carnitine palmitoyltransferase 1a (liver) | ||
| Cpt2 | CAAAAGACTCATCCGCTTTGTTC | CATCACGACTGGGTTTGGGTA |
| Carnitine palmitoyltransferase 2 | ||
| Cyp4a14 | TCATGGCGGACTCTGTCAATA | GCAGGCGAAAGAAAGTCAGG |
| Cytochrome P450, family 4, subfamily a, polypeptide 14 | ||
| Ehhadh | ACAGCGATACCAGAAGCCAG | TGGCAATCCGATAGTGACAGC |
| Enoyl-coenzyme A, hydratase/3-hydroxyacyl coenzyme A dehydrogenase | ||
| Fabp1 | AAGGCAGTCGTCAAGCTGG | CATTGAGTTCAGTCACGGACTT |
| Fatty acid bind protein 1 (liver) | ||
| Fgf21 | CTGCTGGGGGTCTACCAAG | CTGCGCCTACCACTGTTCC |
| Fibroblast growth factor 21 | ||
| Gck | AGACGAAACACCAGATGTATTCC | GAAGCCCTTGGTCCAGTTGAG |
| Glucokinase | ||
| Nr3c1 | CCGGGTCCCCAGGTAAAGA | TGTCCGGTAAAATAAGAGGCTTG |
| Glucocorticoid receptor | ||
| Hadha | AGCAACACGAATATCACAGGAAG | AGGCACACCCACCATTTTGG |
| Hydroxyacyl-coenzyme A dehydrogenase, alpha subunit | ||
| Hadhb | TGAATATGCACTGCGTTCTCAT | CCTTTCCTGGTACTTTGAAGGG |
| Hydroxyacyl-coenzyme A dehydrogenase, beta subunit | ||
| Hk1 | CGGAATGGGGAGCCTTTGG | GCCTTCCTTATCCGTTTCAATGG |
| Hexokinase 1 | ||
| Fkbp51 | TTTGAAGATTCAGGCGTTATCCG | GGTGGACTTTTACCGTTGCTC |
| FK506 binding protein 51 | ||
| Pex19 | GACAGCGAGGCTACTCAGAG | GCCCGACAGATTGAGAGCA |
| Peroxisomal biogenesis factor 19 | ||
| Ppara | TCGGCGAACTATTCGGCTG | GCACTTGTGAAAACGGCAGT |
| Peroxisome proliferator activated receptor alpha | ||
| Slc25a20 | GCGCCCATCATTGGAGTCA | CACACCAGATAACATCCCAGC |
| Solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20 | ||
| 36B4/RplP0 | CGAGGACCGCCTGGTTCTC | GTCACTGGGGAGAGAGAGG |
| Ribosomal protein P0 | ||