(
A) Confocal fluorescence showing the variation of MYC expression in U2OS
Tet-On cells treated with 1 µg/ml doxycycline and ethanol control. Images were taken under the same imaging conditions. The scale bar represents 20 µm (blue: HOECHST, violet: Y69). (
B,
D) Confocal fluorescence images showing the variation of MYC expression in U2OS
Tet-On cells treated with 1 µg/ml doxycycline (
D) and ethanol (
B). The scale bar represents 10 µm. (
C,
E) Kernel density plots showing the quantification of MYC staining as shown in
B and
D. Grey dashed line: log
2FC value defining a region containing 80% of all cells, yellow bar: outlier. (
F) Simulations as in
Figure 3G, but yellow areas here indicate the fold variation of MYC expression in the cell population as calculated from panels (
C) and (
E).