Figure 2.

Circadian Subcellular Localization of PER2::VENUS

(A) Representative confocal images of SCN neurons from brain sections taken from Per2^Venus animals across the LD cycle. PER2::VENUS -positive (green) localization was compared with cytoplasmic immunostaining for AVP (red) and nuclear staining of DAPI (blue). Scale bar, 20 μm.

(B) Co-localization of PER2::VENUS and DAPI assessed by Mander’s coefficient analysis (M1, blue, co-localization of PER2::VENUS with DAPI; M2, yellow, co-localization of DAPI with PER2::VENUS). Note that M2 changes across the day because the overall level of PER2::VENUS changes; thus, the proportion of DAPI nuclei containing PER2::VENUS changes.

(C) Per2^Venus SCN slices were fixed, counterstained with DAPI (blue), and imaged at different time points during the rising phase (CT0–CT9) of the PER2 circadian cycle. Each image shows representative neurons found at that time point, rather than a representative field of view at that time point.

See also Figure S2.