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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Oct 7;98(6):1199–1221. doi: 10.1111/mmi.13209

Figure 2.

Figure 2

Evidence for presence of t6A in tRNAs isolated from E. coli carrying the prfA1 allele (US477) or both prfA1 Δ1->4tsaC alleles (MRA100) based on LC-MS/MS analysis. (left) Analysis of t6A levels in MRA100. Black arrow indicates t6A peak; inset shows extracted ion chromatogram (XIC) corresponding to the molecular ion for t6A. (right) Comparison of relative t6A levels in wild-type and mutant strains. The ratio of Ψ-modified base/m22G were used to normalize the level tRNAs between strains.