Skip to main content
. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Oct 7;98(6):1199–1221. doi: 10.1111/mmi.13209

Figure 4.

Figure 4

Overexpression of rstA does not compensate for the absence of tsaD or tsaC. The chromosomal copies of tsaD (PTET∷tsaD) (A) or tsaC (PTET∷tsaC) (B) have been placed under control of the tightly regulated PTET promoter. For complementation assays, cells were transformed with 100 ng of plasmid (prstA, pBAD24 or pBAD∷tsaD) via electroporation, then recovered with 1 mL of LB and placed at 37°C for 1 hour with shaking. 200 μL of cells were plated on LB-amp supplemented with or without aTc (50 ng/ml) or 0.02% arabinose.