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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Oct 7;98(6):1199–1221. doi: 10.1111/mmi.13209

Figure 6.

Figure 6

HPLC analysis of nucleosides in digests of tRNAs extracted from wild-type and mutant strains of D. radiodurans, S. mutans and Synechocystis sp. PCC 6803. Red line in each panel indicates t6A synthetic standard. (A) D. radiodurans R1: wild-type (black line), ΔtsaD,ΔtsaB (WDZ) (blue line), ΔtsaD (XYD) (green line), ΔtsaB (XYZ) (pink line). (B) S. mutans UA159: wild-type (black line), ΔtsaE (JB409) (blue line), ΔtsaE (ptsaE) (JB409c) (green line). (C) Synechocystis sp. PCC 6803: wild-type (black line) and ΔtsaD (gcp) (blue line).