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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Microbiol. 2015 Oct 7;98(6):1199–1221. doi: 10.1111/mmi.13209

Figure 9.

Figure 9

Representative LC-MS nucleoside analysis for N6-threonylcarbamoyladenosine (t6A) and lysidine (k2C) isolated from total tRNA of D. radiodurans and T. thermophilus. (A) D. radiodurans wild-type and mutant (ΔtsaD) samples. Extracted ion chromatograms (XIC) for m/z range 412.7-413.7 corresponding to the molecular ion for t6A. (B) D. radiodurans ΔtsaD (XYD) XIC for m/z range 371.7-373.7 corresponding to the molecular ion for k2C [(M=H)+ = 372 Da]. Similar results were found in the D. radiodurans wild-type. (C) MS/MS spectrum obtained from RNase T1 digestion of tRNA from T. thermophilus wild-type and (D) Δttha0793 (ΔtsaC) MS/MS verification of the presence or absence of k2C and t6A in tRNAIleCAU.