Skip to main content
. Author manuscript; available in PMC: 2017 Aug 27.
Published in final edited form as: Nat Prod Rep. 2016 Apr 13;33(8):963–987. doi: 10.1039/c6np00017g

Fig. 7. Chemically inducible chromosomal evolution (CIChE) enables stable high-level expression of heterologous genes without selection.

Fig. 7

CIChE enables the creation of multiple chromosomal copies of a recombinant allele based on recA-mediated crossover between leading and trailing homologous regions on different DNA strands. The process is terminated by recA deletion. Reproduced with permissions.279