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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Innate Immun. 2016 Jun 1;22(6):433–443. doi: 10.1177/1753425916651985

Figure 6.

Figure 6

Neonatal naïve B cells demonstrate impaired IL-21 induced class-switching and expression of co-stimulatory receptors CD80 and CD86. Naïve B cells were stimulated with IL-2 (30 ng/mL) and CD40L (20 ng/mL) alone, or in combination with R848 (10 μM), CpG (1 μM) and/or IL-21 (50 ng/ml). Cells were cultured with stimulation for 80 hours before cells were transferred to ELISpot assay plates (104 cells per well for IgM, 105 for IgG). (A) IgG and IgM spots from naïve B cells, with stimulation conditions as indicated above. (B-D) Quantification of surface expression of CD25, CD80 and CD86 by flow cytometry. Fluorescence intensities are shown as MFI fold increase over the control condition. N=4 for all conditions. 2-tailed, unpaired Student’s t-tests for comparison between adults and newborns is indicated. * p<0.05, ** p<0.01, *** p<0.001